LPO 抗体 (Biotin)
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北京 101111
Quick Overview for LPO 抗体 (Biotin) (ABIN457588)
抗原
See all LPO 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Bovine lactoperoxidase, conjugated with Biotin
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特异性
- Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine lactoperoxidase.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Bovine Serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, but reactivity with lactoperoxidase from Goat and Sheep may be expected.
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产品特性
- The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.
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纯化方法
- Purified
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免疫原
- Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It sis isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lactoperoxidase (LPO) (AA 351-589) antibody
LPO 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 305-498) antibodyLPO 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 244-346) antibodyLPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 351-589) antibodyLPO 适用: 大鼠 WB 宿主: 小鼠 Monoclonal 15# unconjugated
anti-Lactoperoxidase (LPO) antibody (Biotin)LPO 适用: Cow WB, IP, ELISA, DB 宿主: 绵羊 Polyclonal Biotin
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- LPO (Lactoperoxidase (LPO))
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别名
- Lactoperoxidase
抗原
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