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CD20 抗体 (Biotin)

This anti-CD20 antibody is a 小鼠 单克隆 antibody detecting CD20 in FACS, IP 和 IHC (fro). Suitable for 人 和 Non-Human Primate. This Primary Antibody has been cited in 6+ publications.
Pubmed (6 references)
产品编号 ABIN775712
发货至: 中国

Quick Overview for CD20 抗体 (Biotin) (ABIN775712)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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人, Non-Human Primate

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD20 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

2H7
Discover our top product MS4A1 Primary Antibody

  • 原理

    Anti-Hu CD20 Biotin

    特异性

    The mouse monoclonal antibody 2H7 recognizes an extracellular epitope on CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

    交叉反应 （详细）

    Human, Non-Human Primates

    纯化方法

    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

    免疫原

    Human tonsillar B cells

    亚型

    IgG2b kappa
  • anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody

    Verified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

    MS4A1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal L26 unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibody

    MS4A1 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

    MS4A1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody

    MS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

    MS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

    MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

    MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody

    MS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibody
    anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibody

    MS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated

  • 应用备注

    Flow cytometry: Recommended dilution: 1 μg/mL.

    限制

    仅限研究用

  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.
    Avoid prolonged exposure to light.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Diehl, Schmidlin, Nagasawa, van Haren, Kwakkenbos, Yasuda, Beaumont, Scheeren, Spits: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, (2008) (PubMed).

    Polyak, Ayer, Szczepek, Deans: "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody." in: Leukemia, Vol. 17, Issue 7, pp. 1384-9, (2003) (PubMed).

    Chan, Hughes, French, Tutt, Walshe, Teeling, Glennie, Cragg: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, (2003) (PubMed).

    Rose, Smith, Maloney: "Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects." in: Blood, Vol. 100, Issue 5, pp. 1765-73, (2002) (PubMed).

    Deans, Robbins, Polyak, Savage: "Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 344-8, (1998) (PubMed).

    Deans, Kalt, Ledbetter, Schieven, Bolen, Johnson: "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22632-8, (1995) (PubMed).

  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    CD20

    背景

    MS4A1,CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.,B1, S7, MS4A, Bp35, CVID5, LEU-16

    基因ID

    931

    UniProt

    P11836
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