CD20 抗体
(1 reference)-
- 抗原 See all CD20 (MS4A1) 抗体
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD20 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes a protein of 30-33 kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
- 无交叉反应
- 牛, 犬, 猪, 大鼠
- 交叉反应 （详细）
- Human. Baboon. Monkey. Does not react with Cow, Dog, Pig or Rat.
- 免疫原
- Human tonsil B cells (L26), Recombinant human MS4A1 protein (IGEL/773)
- 克隆位点
- L26-IGEL-773
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product MS4A1 Primary Antibody
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人 FACS, WB, IF, IHC, StM 宿主: 小鼠 Monoclonal L26 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibodyMS4A1 适用: 人 FACS, WB, IF, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibodyVerified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 WB, IF, IHC 宿主: 兔 Monoclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibodyMS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (5-10 µL/million cells), ,Immunofluorescence (1:50-1:100), ,Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Daudi, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.
- 限制
- 仅限研究用
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- 缓冲液
- Tissue culture supernatant with 0.05% Azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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: "Industrial Processing Induces Pericardial Patch Degeneration." in: Frontiers in surgery, Vol. 9, pp. 881433, (2022) (PubMed).
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: "Industrial Processing Induces Pericardial Patch Degeneration." in: Frontiers in surgery, Vol. 9, pp. 881433, (2022) (PubMed).
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- 抗原
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
- 别名
- MS4A1 (MS4A1 产品)
- 别名
- B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
- 背景
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APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1 (MS4A1),CD20 / MS4A1 (B-Cell Marker)
Cellular localisation: Predominantly cell surface with some cytoplasmic - 分子量
- 33-37kDa
- 基因ID
- 931, 712553
- UniProt
- P11836
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