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Keratin Basic 抗体

适用: 人 WB, IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal AE3 unconjugated
产品编号 ABIN768698
发货至: 中国
  • 抗原 See all Keratin Basic 抗体
    Keratin Basic
    适用
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    • 8
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    宿主
    • 12
    • 9
    小鼠
    克隆类型
    • 21
    单克隆
    标记
    • 13
    • 1
    • 1
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    • 1
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    非结合性
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    品牌
    IHC-plus™
    特异性
    This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
    纯化方法
    Purified IgG
    免疫原
    Human epidermal cells.

    Type of Immunogen: Cells
    克隆位点
    AE3
    亚型
    IgG1
    Top Product
    Discover our top product Primary Antibody
  • 应用备注
    Approved: IHC, IHC-Fr, IHC-P (1:100), WB (1:100)

    Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37 °C. Possibly, higher dilutions can be used if sections are incubated at 4 °C overnight. For use in Western blotting a dilution of 1:100 is advised.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    0.01M PBS containing 0.1 % Sodium azide and 1 % BSA. Sourced in Ascites.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store at 4°C.
  • 抗原
    Keratin Basic
    别名
    Basic Cytokeratin (Keratin Basic 产品)
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