Keratin Basic 抗体
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北京 101111
Quick Overview for Keratin Basic 抗体 (ABIN768698)
抗原
See all Keratin Basic 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
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纯化方法
- Purified IgG
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免疫原
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Human epidermal cells.
Type of Immunogen: Cells -
亚型
- IgG1
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anti-Keratin Basic antibody
Carrier-free 适用: 人, 大鼠 IHC (p) 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人 IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibodyCarrier-free 适用: 人 IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
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应用备注
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Approved: IHC, IHC-Fr, IHC-P (1:100), WB (1:100)
Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37 °C. Possibly, higher dilutions can be used if sections are incubated at 4 °C overnight. For use in Western blotting a dilution of 1:100 is advised. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.01M PBS containing 0.1 % Sodium azide and 1 % BSA. Sourced in Ascites.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C.
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- Keratin Basic
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别名
- Basic Cytokeratin
抗原
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