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Caspase 3 抗体 (Internal Region)

Verified CASP3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN768625
发货至: 中国
  • 抗原 See all Caspase 3 (CASP3) 抗体
    Caspase 3 (CASP3)
    抗原表位
    • 19
    • 16
    • 16
    • 16
    • 16
    • 16
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    • 15
    • 14
    • 13
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    • 2
    • 2
    • 2
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    • 1
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    • 1
    • 1
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    • 1
    • 1
    • 1
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    Internal Region
    适用
    • 272
    • 191
    • 147
    • 31
    • 17
    • 10
    • 9
    • 9
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 275
    • 39
    • 4
    • 2
    • 1
    山羊
    克隆类型
    • 272
    • 48
    多克隆
    标记
    • 152
    • 26
    • 21
    • 14
    • 11
    • 11
    • 9
    • 9
    • 9
    • 9
    • 9
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    This Caspase 3 antibody is un-conjugated
    应用范围
    • 246
    • 107
    • 104
    • 85
    • 79
    • 65
    • 48
    • 34
    • 29
    • 28
    • 25
    • 19
    • 10
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    原理
    Caspase 3
    序列
    RDVSKEDHSK RS
    特异性
    This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
    交叉反应
    犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2, NP_116786.1.
    亚型
    IgG
    Top Product
    Discover our top product CASP3 Primary Antibody
  • 应用备注

    Western Blot: Approx 32 kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6 kDa according to Human NP_004337.2 and 31.4 kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65 kDa was also

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Zhào, Liu, Zeng, Li, Zhang, Huang: "Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).

  • 抗原
    Caspase 3 (CASP3)
    别名
    CASP3 (CASP3 产品)
    别名
    CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody
    背景
    CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3
    分子量
    31.6kDa according to NP_004337.2
    基因ID
    836
    NCBI登录号
    NP_004337, NP_116786
    途径
    Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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