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SMAD5 抗体 (pSer463, Ser465)

This 兔 单克隆 antibody specifically detects SMAD5 in WB, IHC, ELISA, IF 和 IP. It exhibits reactivity toward 人, 大鼠 和 小鼠.
产品编号 ABIN8124746
发货至: 中国
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Quick Overview for SMAD5 抗体 (pSer463, Ser465) (ABIN8124746)

抗原

See all SMAD5 抗体
SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

适用

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人, 大鼠, 小鼠

宿主

  • 154
  • 15
兔

克隆类型

  • 104
  • 65
单克隆

标记

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This SMAD5 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

AB1609
  • 抗原表位

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    pSer463, Ser465

    原理

    SMAD5 (Phospho Ser463/Ser465) Rabbit mAb

    特异性

    The antibody detects endogenous levels of SMAD5 protein only when phosphorylated at Ser463 and Ser465. The antibody does not cross-react with SMAD5 phosphorylated at other sites.

    纯化方法

    The antibody was purified by protein A affinity chromatography.

    免疫原

    Recombinant protein (or fragment).

    亚型

    IgG
  • 应用备注

    WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid freeze / thaw cycles

    储存条件

    -20 °C

    储存方法

    Store at-20°C. Avoid freeze / thaw cycles.
  • 抗原

    SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

    别名

    SMAD5

    背景

    Synonyms: SMAD5, MADH5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5

    Background: The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

    Gene Name: SMAD5

    Protein Name: SMAD5 (Phospho Ser463/Ser465)

    基因ID

    4090

    UniProt

    Q99717
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