RPA2 抗体
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北京 101111
Quick Overview for RPA2 抗体 (ABIN8124318)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- RPA32/RPA2 Rabbit mAb
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特异性
- This antibody detects endogenous levels of RPA32/RPA2 protein.
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纯化方法
- The antibody was purified by protein A affinity chromatography.
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免疫原
- Recombinant protein (or fragment).
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亚型
- IgG
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应用备注
- WB, 1:1000-1:5000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze / thaw cycles
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储存条件
- -20 °C
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储存方法
- Store at-20°C. Avoid freeze / thaw cycles.
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA32/RPA2
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背景
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Synonyms: RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
Background: function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. , PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis) . Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. , subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. , subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. ,
Gene Name: RPA2
Protein Name: RPA32/RPA2
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基因ID
- 6118
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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