Pyruvate Dehydrogenase E1 alpha 抗体
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Quick Overview for Pyruvate Dehydrogenase E1 alpha 抗体 (ABIN8124176)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Pyruvate Dehydrogenase E1 α Rabbit mAb
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特异性
- This antibody detects endogenous levels of Pyruvate Dehydrogenase E1 α protein.
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纯化方法
- The antibody was purified by protein A affinity chromatography.
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免疫原
- Recombinant protein (or fragment).
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亚型
- IgG
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anti-Pyruvate Dehydrogenase E1 alpha (Center) antibody
适用: 人, 小鼠, 大鼠 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase E1 alpha (pSer232) antibody适用: 人 DB, WB 宿主: 兔 Polyclonal RB29473 unconjugated
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应用备注
- WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze / thaw cycles
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储存条件
- -20 °C
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储存方法
- Store at-20°C. Avoid freeze / thaw cycles.
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- Pyruvate Dehydrogenase E1 alpha
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背景
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Synonyms: PDHA1, PHE1A, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I
Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) , and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1) , dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],
Gene Name: >>Glycolysis / Gluconeogenesis, >>Citrate cycle (TCA cycle) , >>Pyruvate metabolism, >>Metabolic pathways, >>Carbon metabolism, >>HIF-1 signaling pathway, >>Glucagon signaling pathway, >>Central carbon metabolism in cancer, >>Diabetic cardiomyopathy
Protein Name: Pyruvate Dehydrogenase E1 α
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基因ID
- 5160
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UniProt
- P08559
抗原
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