Pyruvate Dehydrogenase E1 alpha (AA 39-295) 抗体
适用: 人
WB, IF
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Pyruvate Dehydrogenase E1 alpha
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抗原表位
- AA 39-295
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha
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anti-Pyruvate Dehydrogenase E1 alpha (Center) antibody
适用: 人, 小鼠, 大鼠 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- Biohazard Informations: This product contains thimerosal which is hazardous.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- 抗原
- Pyruvate Dehydrogenase E1 alpha
- 背景
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The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4).The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]
- 分子量
- 43 kDa
- 基因ID
- 5160
- NCBI登录号
- NM_000284, NP_000275
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