EIF2A 抗体 (pSer51)
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Quick Overview for EIF2A 抗体 (pSer51) (ABIN8121299)
抗原
See all EIF2A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer51
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原理
- eIF2α (Phospho Ser51) mouse mAb
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特异性
- This antibody detects endogenous levels of eIF2α (Phospho Ser51) protein.
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纯化方法
- The antibody was purified by protein G affinity chromatography.
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免疫原
- A synthesized phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha.
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亚型
- IgG
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应用备注
- WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze / thaw cycles
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储存条件
- -20 °C
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储存方法
- Store at-20°C. Avoid freeze / thaw cycles.
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- eIF2alpha
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背景
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Synonyms: Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha) , EIF2S1, EIF2A
Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1) , the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908) , and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. , 1987 [PubMed 2948954]) . [supplied by OMIM, Feb 2010],
Gene Name: EIF2S1
Protein Name: eIF2α
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基因ID
- 1965
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UniProt
- P05198
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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