Recombinant Perforin 1 抗体 (AA 400-555)
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Quick Overview for Recombinant Perforin 1 抗体 (AA 400-555) (ABIN8093400)
抗原
See all Perforin 1 (PRF1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 400-555
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原理
- Perforin Antibody / PRF1 (azide and preservative free)
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免疫原
- A recombinant fragment (around amino acids 400-555) of human Perforin-1 protein (exact sequence is proprietary) was used as the immunogen for the Cytotoxic Lymphocyte Perforin/PRF1 antibody.
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亚型
- IgG, kappa
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anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 280-555) antibody
PRF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, Cow FACS, IP, ICC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal dG9 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 461-555) antibodyPRF1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 413-552) antibodyPRF1 适用: 人 IHC 宿主: 小鼠 Monoclonal PRF1-2470 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 40-355) antibodyPRF1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 APC
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (FITC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 FITC
anti-Perforin 1 (Pore Forming Protein) (PRF1) (N-Term) antibodyPRF1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 21-554) antibodyPRF1 适用: 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the Cytotoxic Lymphocyte Perforin/PRF1 antibody should be determined by the researcher.
2. This Cytotoxic Lymphocyte Perforin/PRF1 antibody is recombinantly produced by expression in CHO cells. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Cytotoxic Lymphocyte Perforin/PRF1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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别名
- Perforin
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背景
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Cytotoxic Lymphocyte Perforin Antibody recognizes Perforin, a calcium-dependent pore-forming protein encoded by the PRF1 gene. Perforin is a critical effector molecule of cytotoxic T lymphocytes and natural killer cells, where it is synthesized, stored in secretory granules, and released upon immune synapse formation. Following antigen recognition, Perforin is exocytosed and inserts into the membrane of target cells, forming transmembrane pores that allow granzymes to enter and initiate apoptosis. This tightly regulated mechanism is central to antiviral defense, tumor immunosurveillance, and elimination of intracellular pathogens.
Perforin belongs to the membrane attack complex/perforin (MACPF) protein family and contains a conserved MACPF domain responsible for pore formation. The protein also includes calcium-binding regions that regulate its conformational change and membrane insertion. Under resting conditions, Perforin remains sequestered within cytotoxic granules to prevent unintended tissue damage. Upon activation of cytotoxic lymphocytes, controlled secretion ensures targeted killing of infected or transformed cells while minimizing collateral injury to surrounding tissues.
The PRF1 gene is located on chromosome 10 and is predominantly expressed in activated CD8-positive T cells and natural killer cells. In lymphoid organs such as spleen, tonsil, and lymph node, Perforin-positive cells are typically scattered within germinal centers and interfollicular regions, reflecting the distribution of activated cytotoxic lymphocytes. Cytoplasmic granular staining is the expected localization pattern in immunohistochemistry due to storage within lytic granules.
Genetic mutations in PRF1 are associated with familial hemophagocytic lymphohistiocytosis and other immune dysregulation syndromes, highlighting the essential role of Perforin in maintaining immune homeostasis. Altered expression levels have also been studied in cancer immunology, transplant rejection, and autoimmune disease research. Quantifying Perforin expression can provide insight into cytotoxic immune activity within tumor microenvironments or inflammatory lesions.
This Cytotoxic Lymphocyte Perforin Antibody (clone PRF1/9681R) is a recombinant monoclonal antibody suitable for detecting PRF1 expression in research applications. A Cytotoxic Lymphocyte Perforin Antibody supports studies of cell-mediated cytotoxicity, immune activation status, and functional characterization of cytotoxic lymphocyte populations in normal and diseased tissues. -
UniProt
- P14222
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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