Recombinant MCM4 抗体 (AA 600-800)
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北京 101111
Quick Overview for Recombinant MCM4 抗体 (AA 600-800) (ABIN8093330)
抗原
See all MCM4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 600-800
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原理
- MCM4 Antibody / DNA replication licensing factor MCM4 (azide and preservative free)
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免疫原
- A recombinant fragment (around amino acids 600-800) of human MCM4 protein (exact sequence is proprietary) was used as the immunogen for the recombinant MCM4/Cell proliferation marker MCM4 antibody.
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亚型
- IgG, kappa
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anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 1-863) antibody
MCM4 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 1-863) antibodyMCM4 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 361-677) antibodyMCM4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) antibodyMCM4 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 2-141) antibodyMCM4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 750-800) antibodyMCM4 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 325-375) antibodyMCM4 适用: 人 IHC 宿主: 山羊 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 325-375) antibodyMCM4 适用: 人, 小鼠 WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 750-800) antibodyMCM4 适用: 人, 小鼠 WB, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Deficient 4 (MCM4) antibodyMCM4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB4960 unconjugated Recombinant Antibody
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应用备注
- Optimal dilution of the recombinant MCM4/Cell proliferation marker MCM4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- MCM4/Cell proliferation marker MCM4 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
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别名
- MCM4
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背景
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MCM4/Cell proliferation marker MCM4 Antibody recognizes Minichromosome maintenance complex component 4, also known as DNA replication licensing factor 4 and commonly described as a cell proliferation marker MCM4 in pathology literature. The MCM4 gene encodes a nuclear protein that forms part of the heterohexameric MCM2-7 complex, the core replicative helicase responsible for unwinding DNA during S phase. Because of its central role in replication licensing and DNA synthesis, MCM4 antibody is widely used as a sensitive indicator of proliferating cells in normal and neoplastic tissues.
MCM4 is expressed in the nuclei of cells that are actively cycling, including those in G1, S, G2, and M phases, but is absent in quiescent or terminally differentiated cells. This expression pattern makes cell proliferation marker MCM4 antibody particularly useful for identifying growth fractions in tumors. Compared with traditional proliferation markers, MCM4 often highlights a broader population of cycling cells, reflecting its role in replication origin licensing before DNA synthesis begins.
At the molecular level, MCM4 interacts with MCM2, MCM3, MCM5, MCM6, and MCM7 to assemble the functional helicase complex. Activation of this complex requires sequential phosphorylation events mediated by cyclin-dependent kinases and DDK, enabling origin firing and fork progression. Dysregulation of MCM4 expression has been associated with genomic instability and tumor progression in several malignancies, including colorectal carcinoma, breast carcinoma, lung carcinoma, and gynecologic cancers. Elevated nuclear labeling with MCM4 antibody frequently correlates with high proliferative index and aggressive clinicopathologic features.
In immunohistochemistry, MCM4 antibody produces distinct nuclear staining in proliferating epithelial, lymphoid, and mesenchymal cells, while non-dividing stromal or differentiated cells typically remain negative. This selective nuclear localization aligns with its biological function in DNA replication. Clone MCM4/13209R is designed to detect endogenous MCM4 protein in research applications and provides clear nuclear labeling suitable for evaluating proliferative activity in formalin-fixed, paraffin-embedded tissue sections.
By targeting a key replication licensing factor, MCM4 antibody serves as a valuable tool for studying cell cycle regulation, oncogenesis, and tissue growth dynamics. Its established role as a cell proliferation marker MCM4 makes it particularly relevant for cancer research and for comparative analyses of proliferative indices across different tissue types and tumor grades. -
UniProt
- P33991
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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