Recombinant HLA-DRA 抗体 (AA 1-200)
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Quick Overview for Recombinant HLA-DRA 抗体 (AA 1-200) (ABIN8093243)
抗原
See all HLA-DRA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-200
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原理
- HLA-DRA Antibody / Major histocompatibility complex class II DR alpha chain
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免疫原
- A recombinant fragment (around amino acids 1-200) of human HLA-DR protein (exact sequence is proprietary) was used as the immunogen for the HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody.
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亚型
- IgG, kappa
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anti-HLA Class II DR alpha (HLA-DRA) (AA 26-254) antibody
HLA-DRA 适用: 人 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1C11A5 unconjugated
anti-HLA Class II DR alpha (HLA-DRA) (AA 26-216) antibodyHLA-DRA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DR alpha (HLA-DRA) (AA 27-216) antibodyHLA-DRA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DR alpha (HLA-DRA) (AA 26-254) antibodyHLA-DRA 适用: 人 WB, FACS, IHC 宿主: 小鼠 Monoclonal 5B13F7 unconjugated
anti-HLA Class II DR alpha (HLA-DRA) (AA 26-254) antibodyHLA-DRA 适用: 人 WB, FACS, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DR alpha (HLA-DRA) (AA 26-254) antibodyHLA-DRA 适用: 人 WB, FACS, IHC 宿主: 小鼠 Monoclonal 8I10H1 unconjugated
anti-HLA Class II DR alpha (HLA-DRA) antibodyHLA-DRA 适用: 人 FACS, IHC, IF 宿主: 小鼠 Monoclonal 19-26-1-same as MB-26-1 unconjugated
anti-HLA Class II DR alpha (HLA-DRA) antibodyHLA-DRA 适用: 人 IHC 宿主: 兔 Monoclonal 470R unconjugated Recombinant Antibody
anti-HLA Class II DR alpha (HLA-DRA) (AA 1-254) antibodyHLA-DRA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DR alpha (HLA-DRA) antibodyHLA-DRA 适用: 人, Cow, Cat FACS, IF 宿主: 小鼠 Monoclonal 169-1B5-2 unconjugated
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应用备注
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1. Optimal dilution of the HLA-DRA Antibody for IHC / HLA-DR alpha immunohistochemistry Antibody should be determined by the researcher.
2. This HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- HLA-DRA (HLA Class II DR alpha (HLA-DRA))
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别名
- HLA-DRA
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背景
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Human leukocyte antigen DR alpha (HLA-DRA) is a major histocompatibility complex class II protein encoded by the HLA-DRA gene and forms the alpha chain of the HLA-DR antigen complex involved in antigen presentation. This molecule pairs with the HLA-DR beta chain to form a heterodimeric receptor that presents processed peptide antigens to CD4-positive T lymphocytes, playing a critical role in adaptive immune responses. HLA-DRA is therefore a central component of the MHC class II antigen presentation pathway and is highly expressed in professional antigen-presenting cells including B lymphocytes, dendritic cells, macrophages, and certain activated epithelial and immune cells.
HLA-DRA Antibody Recombinant Rabbit MAb for IHC is used to detect expression of the HLA-DR alpha chain in formalin-fixed, paraffin-embedded tissues using immunohistochemistry. In normal tissues, HLA-DR alpha expression is typically observed in antigen-presenting cells within lymphoid organs and inflammatory microenvironments. Immunohistochemical staining often reveals membranous and cytoplasmic signal in B cells, macrophages, dendritic cells, and other immune cell populations involved in antigen processing and presentation. Detection of HLA-DRA by immunohistochemistry therefore provides valuable information about immune activation, antigen presentation, and inflammatory responses within tissue samples.
The HLA-DR antigen is widely used as a marker of immune activation and antigen-presenting cell identity in both normal and diseased tissues. Because the HLA-DR complex consists of alpha and beta chains encoded by separate genes, antibodies targeting the alpha chain such as HLA-DRA antibody reagents allow specific evaluation of this component of the MHC class II receptor. Expression of HLA-DR alpha is tightly regulated and can be induced by cytokines such as interferon-gamma during immune responses. As a result, HLA-DRA expression frequently increases in inflammatory conditions, infection, autoimmune disease, and tumor microenvironments where immune cell infiltration occurs.
In diagnostic and research pathology, HLA-DRA antibody staining is frequently used to evaluate immune cell populations and antigen presentation activity within tissues. The HLA-DR alpha chain is commonly detected in lymphoid tissues, sites of inflammation, and tumors with significant immune infiltration. Because HLA-DR expression reflects activation of antigen-presenting pathways, immunohistochemical analysis of HLA-DRA can help characterize immune microenvironments and assess interactions between immune cells and tumor cells. Clone MSVA-470R is a recombinant rabbit monoclonal antibody designed to recognize HLA-DRA and supports detection of the HLA-DR alpha chain in immunohistochemical staining applications.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P01903
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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