Recombinant Elastase 3B 抗体 (AA 82-238)
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Quick Overview for Recombinant Elastase 3B 抗体 (AA 82-238) (ABIN8093147)
抗原
See all Elastase 3B (ELA3B) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 82-238
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原理
- CELA3B Antibody / Chymotrypsin like elastase 3B
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免疫原
- Recombinant human CELA3B protein fragment (around amino acids 82-238) (exact sequence is proprietary) was used as the immunogen for the CELA3B antibody for IHC.
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亚型
- IgG1, kappa
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anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibody
ELA3B 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal CELA3B-1257 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal CELA3B-1757 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC 宿主: 小鼠 Monoclonal 410M unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal rCELA3B-1811 unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC 宿主: 兔 Monoclonal CELA3B-2809R unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 70-98), (N-Term) antibodyELA3B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33872 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 28-267) antibodyELA3B 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 19-270) antibodyELA3B 适用: 人 WB 宿主: 小鼠 Monoclonal 3H3 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyCarrier-free ELA3B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal CELA3B-1257 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal CELA3B-1758 unconjugated
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应用备注
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1. Optimal dilution of the CELA3B antibody for IHC should be determined by the researcher.
2. This CELA3B/Chymotrypsin like elastase 3B antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- CELA3B/Chymotrypsin like elastase 3B antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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别名
- CELA3B
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背景
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CELA3B Antibody for IHC recognizes Chymotrypsin-like elastase family member 3B, a secreted digestive serine protease commonly referred to as pancreatic elastase 3B or elastase 3B. The human CELA3B gene is located on chromosome 1p36.12 and encodes a protein that is highly expressed in pancreatic acinar cells, where it is synthesized, processed, and stored within zymogen granules prior to secretion into the duodenum. As a member of the chymotrypsin family of serine proteases, CELA3B plays an important role in dietary protein digestion through regulated proteolytic activity in the intestinal lumen.
Chymotrypsin-like elastase family member 3B belongs to the elastase subfamily of trypsin-like proteases and shares strong structural homology with CELA3A. The enzyme is produced as a preproprotein containing a signal peptide that directs entry into the secretory pathway, followed by an activation peptide that maintains the protease in an inactive state until cleavage by trypsin. The mature enzyme adopts the conserved serine protease fold with a catalytic triad that mediates peptide bond hydrolysis. Because of its secretory routing, CELA3B localizes to the endoplasmic reticulum and Golgi during biosynthesis and is concentrated within cytoplasmic secretory granules of pancreatic acinar cells prior to extracellular release.
CELA3B is sometimes referenced in clinical literature under the term fecal elastase 1, reflecting its stability in stool and its use as a biochemical marker of pancreatic exocrine function. In tissue-based research settings, elastase 3B is widely recognized as a lineage-associated marker of acinar differentiation. Immunohistochemical studies have demonstrated strong expression in normal pancreatic acinar cells, while comparative tumor profiling supports its relevance in the evaluation of pancreatic acinar cell carcinoma and other exocrine pancreatic neoplasms. These characteristics make CELA3B a useful target in research panels focused on pancreatic development, differentiation, and tumor classification.
Genetic investigations have also linked CELA3B variants to inherited or familial pancreatitis phenotypes, underscoring its biological importance in digestive enzyme regulation and pancreatic homeostasis. Dysregulation of digestive protease activation is a central mechanism in pancreatitis pathogenesis, and CELA3B contributes to the tightly controlled proteolytic environment of the exocrine pancreas. The recombinant mouse monoclonal clone MSVA-410M is designed to detect CELA3B expression in formalin-fixed, paraffin-embedded tissue samples, supporting research applications centered on pancreatic acinar biology and elastase family protein expression.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.
Researchers studying pancreatic differentiation, exocrine digestive enzyme biology, and acinar cell-associated tumor pathways may also be interested in our < a href=../tds/elastase-3b-antibody-cela3b-cela3b1257-v3436>Elastase 3B Antibody / Pancreatic Digestive Enzyme page featuring validated immunohistochemistry and western blot applications for pancreatic research. -
UniProt
- P08861
抗原
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