Recombinant TdT 抗体 (N-Term)
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Quick Overview for Recombinant TdT 抗体 (N-Term) (ABIN8093128)
抗原
See all TdT (DNTT) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 52-192, N-Term
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原理
- TdT Antibody / DNA nucleotidylexotransferase
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免疫原
- A recombinant fragment (around amino acids 52-192) of human Terminal deoxynucleotidyl transferase protein (exact sequence is proprietary) was used as the immunogen for the TdT antibody for IHC.
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亚型
- IgG, kappa
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anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 52-192) antibody
DNTT 适用: 人, 大鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4B10A6 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 52-192) antibodyDNTT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B10C3 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) antibodyDNTT 适用: 人 IHC 宿主: 兔 Monoclonal 453R unconjugated Recombinant Antibody
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 1-110) antibodyDNTT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 351-450) antibodyDNTT 适用: 人 FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (Internal Region) antibodyDNTT 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 1-509) antibodyDNTT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) antibodyDNTT 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB1660 unconjugated Recombinant Antibody
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 316-509) antibodyDNTT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) antibodyDNTT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the TdT Antibody for IHC / DNTT Antibody should be determined by the researcher.
2. This DNTT/Terminal deoxynucleotidyl transferase antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- TdT/DNTT antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))
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别名
- TdT
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背景
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TdT Antibody for IHC detects Terminal deoxynucleotidyl transferase (DNTT), a specialized nuclear DNA polymerase encoded by the DNTT gene on chromosome 10q23-q24. Terminal deoxynucleotidyl transferase, commonly referred to as TdT and also known as DNA nucleotidylexotransferase or Terminal transferase, is a member of the DNA polymerase X family selectively expressed in immature lymphoid cells. A TdT Antibody for IHC is commonly used to identify immature lymphoid populations in tissue sections and to support detection of lymphoblastic neoplasms in histological analysis. This recombinant monoclonal antibody clone MSVA-453R enables reliable immunohistochemical detection of nuclear TdT expression in formalin-fixed tissue samples. This antibody is part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.
TdT antibody, also referred to as DNTT antibody and Terminal transferase antibody in the literature, recognizes a nuclear enzyme that catalyzes the template-independent addition of deoxynucleotides to the 3-prime hydroxyl ends of DNA during V(D)J recombination. This unique enzymatic activity introduces N-region nucleotide diversity within immunoglobulin and T cell receptor gene segments, significantly expanding antigen receptor repertoire complexity. TdT functions within nuclear recombination centers in coordination with RAG1 and RAG2 complexes and additional components of the non-homologous end joining pathway. Its catalytic core contains conserved polymerase domains characteristic of the Pol X family, enabling nucleotide incorporation without requiring a DNA template.
DNTT expression is tightly regulated during lymphoid development and is largely restricted to early B and T lymphoblasts in bone marrow and thymus. In the thymic cortex, TdT-positive cells represent developing T cell precursors undergoing receptor rearrangement. Expression declines as lymphocytes mature, making TdT a well-established marker of lymphoid immaturity. A TdT Antibody for IHC typically demonstrates strong nuclear staining in immature lymphoid cells and is widely used to identify leukemic blast populations in acute lymphoblastic leukemia and lymphoblastic lymphoma, where nuclear TdT positivity serves as a key diagnostic feature distinguishing precursor lymphoid malignancies from mature lymphoid and non-hematologic tumors.
Structurally, Terminal deoxynucleotidyl transferase contains regulatory regions that influence substrate selection and protein-protein interactions within recombination complexes. Alternative splicing of DNTT can generate isoforms with subtle biochemical differences that may affect catalytic efficiency and regulation. Persistent or dysregulated expression of DNTT contributes to genomic variability and is implicated in lymphoid malignancy development. Through its central role in immune receptor diversification, TdT remains essential to normal lymphopoiesis and disease-oriented research applications. This TdT Antibody for IHC supports detection of nuclear TdT expression in studies of lymphoid development, leukemia biology, and hematologic malignancy research, and is available from NSJ Bioreagents. It can be compared with our < a href=../tds/tdt-antibody-dna-nucleotidylexotransferase-tdt1393-v8399>TdT Antibody (clone TDT/1393) for detection of terminal deoxynucleotidyl transferase in lymphoid precursor cells and hematologic malignancy studies. -
UniProt
- P04053
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途径
- DNA Damage Repair
抗原
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