Recombinant Decorin 抗体 (AA 212-336)
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Quick Overview for Recombinant Decorin 抗体 (AA 212-336) (ABIN8093127)
抗原
See all Decorin (DCN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 212-336
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原理
- Decorin Antibody / DCN
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免疫原
- Recombinant human Decorin protein fragment (around amino acids 212-336) (exact sequence is proprietary) was used as the immunogen for the Decorin antibody for IHC.
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亚型
- IgG, kappa
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anti-Decorin (DCN) (AA 31-359) antibody
DCN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Decorin (DCN) (AA 122-150) antibodyDCN 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19595 unconjugated
anti-Decorin (DCN) (AA 263-324) antibodyDCN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5E8E7 unconjugated
anti-Decorin (DCN) (AA 1-359) antibodyDCN 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3H4-1F4 unconjugated
anti-Decorin (DCN) antibodyDCN 适用: 人 WB, IHC (p), IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Decorin (DCN) (C-Term) antibodyDCN 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Decorin (DCN) (AA 31-359) antibodyDCN 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Decorin (DCN) antibodyDCN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Decorin (DCN) (AA 212-336) antibodyDCN 适用: 人 IHC 宿主: 小鼠 Monoclonal DCN-3523 unconjugated
anti-Decorin (DCN) (AA 1-360) antibodyDCN 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3H4-1F4 unconjugated
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应用备注
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1. Optimal dilution of the DCN/Decorin antibody should be determined by the researcher.
2. This DCN antibody for IHC is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
4. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- DCN/Decorin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Decorin (DCN)
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别名
- Decorin
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背景
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Decorin Antibody for IHC detects Decorin (DCN), a small leucine-rich extracellular matrix proteoglycan encoded by the DCN gene and widely distributed in connective tissues. Clone MSVA-537R is a recombinant monoclonal antibody developed for immunohistochemistry and supports visualization of Decorin within stromal extracellular matrix compartments of formalin-fixed tissue sections. This antibody is part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.
Decorin antibody, also referred to as DCN antibody and small leucine-rich proteoglycan Decorin antibody in the literature, recognizes a secreted proteoglycan characterized by tandem leucine-rich repeat domains and a single dermatan sulfate or chondroitin sulfate glycosaminoglycan chain. Decorin binds to fibrillar collagens, particularly type I collagen, where it regulates collagen fibrillogenesis, fiber diameter, and interfibrillar spacing. Through these interactions, Decorin contributes to tissue tensile strength and extracellular matrix stability.
Beyond structural support, Decorin modulates cellular signaling pathways through interactions with growth factors and receptor tyrosine kinases, including transforming growth factor beta and epidermal growth factor receptor. These regulatory effects influence cell proliferation, differentiation, and migration, positioning Decorin as both a matrix scaffold and a signaling modulator within the tissue microenvironment. Decorin expression is prominent in skin, tendon, ligament, placenta, prostate stroma, and other collagen-rich tissues. A Decorin Antibody for IHC typically demonstrates extracellular stromal staining between collagen bundles and surrounding glandular or vascular structures and is commonly used to evaluate extracellular matrix organization, fibrosis, and tumor-associated desmoplastic responses.
Altered Decorin expression has been associated with fibrosis, abnormal wound healing, and tumor progression, where remodeling of collagen architecture and stromal signaling contributes to disease development. Assessment of Decorin distribution may therefore support research into extracellular matrix biology, connective tissue organization, and tumor microenvironment remodeling. This Decorin Antibody for IHC targets Decorin in research applications and is available from NSJ Bioreagents.
This antibody can be compared with our < a href=../tds/decorin-antibody-dcn3521-v5683>Decorin Antibody (clone DCN/3521) for consistent detection of DCN across extracellular matrix and proteoglycan biology studies. -
UniProt
- P07585
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途径
- Glycosaminoglycan Metabolic Process
抗原
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