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SMAD3 抗体 (pSer204)

Name: SMAD3 抗体 (pSer204)
SKU: ABIN756832
Availability: OutOfStock

This anti-SMAD3 antibody is a 兔多克隆 antibody detecting SMAD3 in WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 1 publication.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-Smad3 (Ser204) Polyclonal Antibody, Unconjugated (ABIN756832) at 1:200 followed by conjugation to the secondary antibody and DAB staining (SMAD3 抗体 (pSer204))

IHC

1 image

(1 reference)

产品编号 ABIN756832

发货至: 中国

Quick Overview for SMAD3 抗体 (pSer204) (ABIN756832)

抗原

See all SMAD3 抗体

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This SMAD3 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product SMAD3 Primary Antibody

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pSer204

特异性

This phosphorylation site is homologous to that of Ser204 in Mouse and Rat.

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Sheep,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser204 [AG(p-S)PN]

亚型

IgG
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody
SMAD3 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 3D1 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
KO Validated SMAD3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Ju, Zhang, Zhu, Nie, Xu, Lei et al.: "Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4(+) T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 ..." in: Basic & clinical pharmacology & toxicology, Vol. 120, Issue 6, pp. 560-570, (2016) (PubMed).
抗原

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

别名

Smad3

背景

Synonyms: LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, SMAD family member 3, SMAD 3, Smad3, hSMAD3
Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

基因ID

4088

UniProt

P84022

途径

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy