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PRKAA1/PRKAA2 抗体 (pThr172, pThr183)

Name: PRKAA1/PRKAA2 抗体 (pThr172, pThr183)
SKU: ABIN752368
Availability: OutOfStock

适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, ELISA, IF (cc), IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (PRKAA1/PRKAA2 抗体 (pThr172, pThr183))

IHC (p)

1 image

(4 references)

产品编号 ABIN752368

发货至: 中国

Datasheet as PDF

抗原 See all PRKAA1/PRKAA2 products

PRKAA1/PRKAA2 (AMPK1/AMPK2)
抗原表位
17

17

1

1

1

1

1

1

1

1

1

1

1

1
pThr172, pThr183
适用
23

23

22

17

15

3

2

1
人, 小鼠, 大鼠, Pig, 绵羊
宿主
24

1
兔
Compatible Secondaries
克隆类型
24

1
多克隆
标记
10

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PRKAA1/PRKAA2 antibody is un-conjugated
应用范围
25

13

12

12

6

5

4

4

3

2
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, Pig, 大鼠, 绵羊

预测反应

Dog,Cow,Horse,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172

亚型

IgG
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody
适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Cy7)
适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, IF (cc), FACS, IF (p) 宿主: 兔 Polyclonal Cy7

anti-AMPK1/AMPK2 (AA 14-47), (N-Term) antibody
适用: 小鼠 WB 宿主: 兔 Polyclonal RB43852 unconjugated

anti-AMPK1/AMPK2 (pThr172), (pThr174) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-AMPK1/AMPK2 (Ser1071) antibody
适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-AMPK1/AMPK2 (Center) antibody
适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-AMPK1/AMPK2 (pSer172), (pSer183) antibody
适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Biotin)
适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Cy3)
适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, IF (cc), FACS, IF (p) 宿主: 兔 Polyclonal Cy3

anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Cy5)
适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, IF (cc), FACS, IF (p) 宿主: 兔 Polyclonal Cy5

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Saito, Chikenji, Ozasa, Fujimiya, Yamashita, Gingery, Iba: "PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome." in: Scientific reports, Vol. 7, Issue 1, pp. 16192, (2019) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, (2016) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).
抗原

PRKAA1/PRKAA2 (AMPK1/AMPK2)

别名

AMPK alpha-1/2 (PRKAA1/PRKAA2 产品)

别名

AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody

背景

Synonyms: PRKAA1phospho T172, AMPK alpha 1 + AMPK alpha 2 phospho T183/T172, 5 AMP activated protein kinase alpha 1catalytic subunit, 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1, acetyl CoA carboxylase kinase, AI194361, AI450832, AL024255, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha -1, AMPK 1, AMPK alpha 1 chain, AMPK, AMPK1, AMPKa1, AMPKalpha1, C130083N04Rik, cb116, EC 2.7.11.1, HMG CoA reductase kinase,hormone sensitive lipase kinase, im:7154392, kinase AMPK alpha1, MGC33776, MGC57364, PRKAA 1, PRKAA1, Protein kinase AMP activated alpha 1 catalytic subunit, SNF1-like protein AMPK, wu:fa94C10.
Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

基因ID

5562