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- 抗原 See all PRKAA1/PRKAA2 products
- PRKAA1/PRKAA2 (AMPK1/AMPK2)
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抗原表位
- AA 14-47, N-Term
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRKAA1/PRKAA2 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 预测反应
- Rat, H, Pig
- 纯化方法
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- 免疫原
- This PRKAA1/2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-47 amino acids from the N-terminal region of human PRKAA1/2.
- 克隆位点
- RB43852
- 亚型
- Ig Fraction
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anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody
适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, ELISA, IF (cc), IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Cy7)适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, IF (cc), FACS, IF (p) 宿主: 兔 Polyclonal Cy7
anti-AMPK1/AMPK2 (Ser1071) antibody适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AMPK1/AMPK2 (Center) antibody适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AMPK1/AMPK2 (pSer172), (pSer183) antibody适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 6 months
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- 抗原
- PRKAA1/PRKAA2 (AMPK1/AMPK2)
- 别名
- PRKAA1/2 (PRKAA1/PRKAA2 产品)
- 别名
- AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody
- 背景
- Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE, regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU, however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
- 分子量
- 64009
- UniProt
- Q13131
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