Dihydrofolate Reductase 抗体 (AA 2-187)
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Quick Overview for Dihydrofolate Reductase 抗体 (AA 2-187) (ABIN7985256)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 2-187
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原理
- Anti-Dihydrofolate reductase (DHFR) Antibody
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- Human DHFR shares 90% amino acid (aa) sequence identity with both mouse,rat DHFR.
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产品特性
- Anti-Dihydrofolate reductase (DHFR) Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
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亚型
- IgG
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anti-Dihydrofolate Reductase (DHFR) (AA 88-187) antibody
DHFR 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6G7 unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 2-187) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 9B2 unconjugated Recombinant Antibody
anti-Dihydrofolate Reductase (DHFR) antibodyKD Validated DHFR 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3575 unconjugated Recombinant Antibody
anti-Dihydrofolate Reductase (DHFR) (AA 16-30), (N-Term) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49346 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-187) antibodyDHFR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-50) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 135-164) antibodyDHFR 适用: 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- Dihydrofolate Reductase (DHFR)
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别名
- DHFR
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背景
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Background: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Gene Full Name: dihydrofolate reductase
Sequence Similarities: Belongs to the dihydrofolate reductase family.
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分子量
- 21 kDa
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基因ID
- 1719
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UniProt
- P00374
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途径
- Mitotic G1-G1/S Phases
抗原
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