Dihydrofolate Reductase 抗体 (AA 2-187) (Fluoro594)
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Quick Overview for Dihydrofolate Reductase 抗体 (AA 2-187) (Fluoro594) (ABIN7985250)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-187
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原理
- Anti-Dihydrofolate reductase (DHFR) Antibody Fluoro594 Conjugated
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins
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预测反应
- Human DHFR shares 90% amino acid (aa) sequence identity with both mouse,rat DHFR.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
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亚型
- IgG
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应用备注
- Flow Cytometry, Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
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有效期
- 12 months
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- Dihydrofolate Reductase (DHFR)
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别名
- DHFR
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背景
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Background: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Gene Full Name: dihydrofolate reductase
Sequence Similarities: Belongs to the dihydrofolate reductase family.
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基因ID
- 1719
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UniProt
- P00374
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途径
- Mitotic G1-G1/S Phases
抗原
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