TdT 抗体 (AA 316-509)
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Quick Overview for TdT 抗体 (AA 316-509) (ABIN7984886)
抗原
See all TdT (DNTT) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 316-509
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原理
- Anti-TdT/DNTT Antibody
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins
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预测反应
- Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
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产品特性
- Anti-TdT/DNTT Antibody. Tested in WB applications. This antibody reacts with Human.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
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亚型
- IgG
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))
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别名
- DNTT
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背景
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Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
Gene Full Name: DNA nucleotidylexotransferase
Sequence Similarities: Belongs to the DNA polymerase type-X family.
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分子量
- 58 kDa
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基因ID
- 1791
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UniProt
- P04053
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途径
- DNA Damage Repair
抗原
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