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TdT 抗体 (AA 316-509) (Fluoro594)

The Fluoro594-conjugated 兔 多克隆 anti-TdT antibody (ABIN7984880) specifically detects TdT in FACS. The antibody is reactive with 人 samples.
产品编号 ABIN7984880
发货至: 中国
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Quick Overview for TdT 抗体 (AA 316-509) (Fluoro594) (ABIN7984880)

抗原

See all TdT (DNTT) 抗体
TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

适用

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宿主

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克隆类型

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多克隆

标记

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This TdT antibody is conjugated to Fluoro594

应用范围

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Flow Cytometry (FACS)
  • 抗原表位

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    AA 316-509

    原理

    Anti-TdT/DNTT Antibody Fluoro594 Conjugated

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

    亚型

    IgG
  • 应用备注

    Flow Cytometry, Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

    有效期

    12 months
  • 抗原

    TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

    别名

    DNTT

    背景

    Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

    Gene Full Name: DNA nucleotidylexotransferase

    Sequence Similarities: Belongs to the DNA polymerase type-X family.

    基因ID

    1791

    UniProt

    P04053

    途径

    DNA Damage Repair
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