BUB3 抗体 (AA 50-209)
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北京 101111
Quick Overview for BUB3 抗体 (AA 50-209) (ABIN7973487)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 50-209
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原理
- Anti-BUB3 Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-BUB3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BUB3 recombinant protein (Position: R50-D209).
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亚型
- IgG
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应用备注
- Western blot, 0.25-0.5 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. -
有效期
- 12 months
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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别名
- BUB3
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背景
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Background: BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
Gene Full Name: BUB3 mitotic checkpoint protein
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分子量
- 40 kDa
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基因ID
- 9184
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UniProt
- O43684
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途径
- Maintenance of Protein Location
抗原
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