BUB3 抗体 (Middle Region)
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北京 101111
Quick Overview for BUB3 抗体 (Middle Region) (ABIN3044239)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-Bub3 Antibody
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序列
- DHQLKMHDLN TDQEN
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- identical to the related rat,mouse sequences.
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产品特性
- Anti-Bub3 Antibody. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Bub3, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 267-327) antibody
BUB3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 6-217) antibodyBUB3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) antibodyKD Validated BUB3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3085 unconjugated Recombinant Antibody
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 275-325) antibodyBUB3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 201-230) antibodyBUB3 适用: Drosophila melanogaster WB 宿主: 兔 Polyclonal RB36332 unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) antibodyBUB3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (N-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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别名
- BUB3
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背景
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Background: BUB3 (BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
Gene Full Name: BUB3 mitotic checkpoint protein
Sequence Similarities: Belongs to the WD repeat BUB3 family.
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分子量
- 37 kDa
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基因ID
- 9184
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UniProt
- O43684
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途径
- Maintenance of Protein Location
抗原
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