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Histone H2A 抗体 (C-Term)

The 小鼠 单克隆 anti-Histone H2A antibody (Clone 10E9-D1) (ABIN7938168) specifically detects Histone H2A in WB 和 ChIP. The antibody is reactive with Drosophila melanogaster samples.
产品编号 ABIN7938168
发货至: 中国
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Quick Overview for Histone H2A 抗体 (C-Term) (ABIN7938168)

抗原

See all Histone H2A 抗体
Histone H2A

适用

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Drosophila melanogaster

宿主

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小鼠

克隆类型

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单克隆

标记

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This Histone H2A antibody is un-conjugated

应用范围

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Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

克隆位点

10E9-D1
  • 抗原表位

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    C-Term

    原理

    Histone H2Av antibody (mAb) (Clone 10E9.D1), sample

    纯化方法

    Protein A Chromatography

    免疫原

    This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.

    亚型

    IgG2a
  • 应用备注

    ChIP: 5 μg per ChIP WB: 0.5 - 2 μg/mL dilution

    限制

    仅限研究用
  • 缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    Histone H2A

    背景

    The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.

    分子量

    15 kDa

    NCBI登录号

    NP_524519
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