Histone H2A 抗体 (H2AK119ub)
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北京 101111
Quick Overview for Histone H2A 抗体 (H2AK119ub) (ABIN7938165)
抗原
See all Histone H2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H2AK119ub
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原理
- Histone H2AK119ub antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.
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亚型
- IgG
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anti-Histone H2A (acLys9) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (pSer139) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (H2AK95ac) antibody适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB6080 unconjugated Recombinant Antibody
anti-Histone H2A (pThr120) antibody适用: 人 WB, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (meGln104) antibody适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (ubLys119) antibody适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (AA 1-15) antibody适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (H2AK9ac) antibody适用: 人 ELISA, IF, ICC 宿主: 兔 Monoclonal 3H5 unconjugated Recombinant Antibody
anti-Histone H2A (AA 1-128) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (pThr121) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- DB: 1:1000 WB*: 1:1000 - 1:2000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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缓冲液
- 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Histone H2A
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背景
- In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.
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分子量
- 23 kDa
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NCBI登录号
- NP_003508
抗原
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