TMEM66 抗体 (Intracellular, Lumenal Region)
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Quick Overview for TMEM66 抗体 (Intracellular, Lumenal Region) (ABIN7885623)
抗原
See all TMEM66 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 141-155, Intracellular, Lumenal Region
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原理
- A Rabbit Polyclonal Antibody to TMEM66
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预测反应
- 15 amino acid residues identical,Mouse - 13, human - 11
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF
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亚型
- IgG
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anti-Transmembrane Protein 66 (TMEM66) (AA 33-62), (N-Term) antibody
TMEM66 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB24188 unconjugated
anti-Transmembrane Protein 66 (TMEM66) (AA 141-155), (Intracellular), (Lumenal Region) antibodyTMEM66 适用: 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 66 (TMEM66) (AA 218-267) antibodyTMEM66 适用: 人, 小鼠, 大鼠, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:500
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7237528)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW)
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- TMEM66 (Transmembrane Protein 66 (TMEM66))
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别名
- Store-operated calcium entry-associated regulatory factor
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背景
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Synonyms: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
Description: TMEM66 (Transmembrane protein 66, also known as SOCE-associated regulatory factor, SARAF) is a regulatory protein of cellular Ca2+ homeostasis, encoded by the TMEM66 gene. TMEM66 is an ER resident protein, which responds to cytosolic Ca2+ elevation after ER Ca2+ refilling.TMEM66 is highly conserved in vertebrates. In mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. The TMEM66 structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail1.Store-operated Ca2+ entry (SOCE) is an important Ca2+ influx pathway across plasma membranes in many non-excitable cells. In this pathway, intracellular Ca2+ release through IP3 receptors stimulated by a variety of agonists, results in reduction of Ca2+ concentration in the lumen of the endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling1,2.STIM, Ca2+ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca2+ release-activated Ca2+ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells2,3.Findings show that dysregulation of Ca2+ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders2. Inherited defects in SOCE due to mutations in genes of the Ca2+ release-activated Ca2+ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells3.
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基因ID
- 290796
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UniProt
- Q6AYN2
抗原
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