TMEM66 抗体 (Intracellular, Lumenal Region)
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Quick Overview for TMEM66 抗体 (Intracellular, Lumenal Region) (ABIN7043863)
抗原
See all TMEM66 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 141-155, Intracellular, Lumenal Region
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原理
- A Rabbit Polyclonal Antibody to TMEM66
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- 15 amino acid residues identical,Mouse - 13, human - 11
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产品特性
- Anti-TMEM66 Antibody (ABIN7043863, ABIN7044034 and ABIN7044035) is a highly specific antibody directed against an epitope of rat transmembrane protein 66. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM66 from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF
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亚型
- IgG
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anti-Transmembrane Protein 66 (TMEM66) (AA 33-62), (N-Term) antibody
TMEM66 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB24188 unconjugated
anti-Transmembrane Protein 66 (TMEM66) (AA 218-267) antibodyTMEM66 适用: 人, 小鼠, 大鼠, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:500
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7237528)
Blocking Peptide: (ABIN7237528)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- TMEM66 (Transmembrane Protein 66 (TMEM66))
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别名
- SARAF
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背景
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Synonyms: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
Description: TMEM66 (Transmembrane protein 66, also known as SOCE-associated regulatory factor, SARAF) is a regulatory protein of cellular Ca2+ homeostasis, encoded by the TMEM66 gene. TMEM66 is an ER resident protein, which responds to cytosolic Ca2+ elevation after ER Ca2+ refilling.TMEM66 is highly conserved in vertebrates. In mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. The TMEM66 structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail1.Store-operated Ca2+ entry (SOCE) is an important Ca2+ influx pathway across plasma membranes in many non-excitable cells. In this pathway, intracellular Ca2+ release through IP3 receptors stimulated by a variety of agonists, results in reduction of Ca2+ concentration in the lumen of the endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling1,2.STIM, Ca2+ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca2+ release-activated Ca2+ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells2,3.Findings show that dysregulation of Ca2+ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders2. Inherited defects in SOCE due to mutations in genes of the Ca2+ release-activated Ca2+ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells3.
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基因ID
- 290796
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NCBI登录号
- NM_016127
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UniProt
- Q6AYN2
抗原
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