GPR65 抗体 (Extracellular Loop)
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Quick Overview for GPR65 抗体 (Extracellular Loop) (ABIN7884992)
抗原
See all GPR65 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 72-84, Extracellular Loop
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原理
- A Rabbit Polyclonal Antibody to GPR65 (TDAG8)
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预测反应
- Mouse - 12,13 amino acid residues identical, rat - 11,13 amino acid residues identical.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65
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亚型
- IgG
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anti-G Protein-Coupled Receptor 65 (GPR65) (1st Extracellular Loop), (AA 72-84) antibody
GPR65 适用: 人 IHC, FACS, LCI, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (Extracellular Domain) antibodyGPR65 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (1st Extracellular Loop), (AA 72-84) antibody (PE)GPR65 适用: 人 FACS 宿主: 兔 Polyclonal PE
anti-G Protein-Coupled Receptor 65 (GPR65) antibodyGPR65 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (1st Extracellular Loop), (AA 72-84) antibody (FITC)GPR65 适用: 人 FACS, LCI 宿主: 兔 Polyclonal FITC
anti-G Protein-Coupled Receptor 65 (GPR65) (C-Term) antibodyGPR65 适用: 人, 小鼠, 大鼠, 犬, 马, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) antibodyGPR65 适用: 人, 小鼠 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (3rd Extracellular Domain) antibodyGPR65 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (1st Cytoplasmic Domain) antibodyGPR65 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: 5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:1200
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235434)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- GPR65 (G Protein-Coupled Receptor 65 (GPR65))
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别名
- G-protein coupled receptor 65
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背景
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Synonyms: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
Description: GPR65, also called TDAG8 (T-cell death associated gene 8 protein), is a pH -sensing G-protein coupled receptor (GPCR) activated by acidic extracellular pH through the protonation of several histidine residues in the receptor's sequence. GPR65 is involved in cancer cell metastasis and proliferation, immune cell function, inflammation, and blood vessel formation1.Human GPR65 gene has been mapped to a location that is associated with T cell lymphoma and leukemia abnormalities.GPR65 is primarily expressed in immune cells and leukocyte-rich tissues such as circulating peripheral leukocytes, spleen, thymus, and tonsils. Expression is also detected pain relevant loci such as the dorsal root ganglia neurons and particularly small diameter neurons responsible for nociception1,2.GPR65 has the ability to affect tumor development and growth. Overexpression of GPR65 in Lewis lung carcinoma cells increases tumor growth in mice and may facilitate resistance to acidosis-mediated cell death in vitro through protein kinase A (PKA) and ERK related pathways1.Knockdown of GPR65 in NCI-H460 human non-small cell lung cancer cells decreases cell survival in acidic condition. Another study shows that activation of GPR65 by acidosis can promote evasion of cell apoptosis under glutamine starvation and its overexpression has been reported to transform immortalized mammary epithelial cells1.
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基因ID
- 8477
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UniProt
- Q8IYL9
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途径
- cAMP Metabolic Process
抗原
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