Substance P 抗体
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北京 101111
Quick Overview for Substance P 抗体 (ABIN7884252)
抗原
See all Substance P 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Substance P / HRP Bispecific Monoclonal Antibody (P4C1)
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特异性
- This is a bispecific antibody which recognizes (A) Substance P and (B) Horseradish peroxidase types I, II, VI, IX, X. This antibody recognizes the COOH-terminal end of substance P. It does not recognize Leu- or Met enkephalin, somatostatin or beta-endorphin, cross-reactivity with eledoisin: 5 % . It recognizes Substance P in post-mortem tissue of the human brain.
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预测反应
- All Mammals
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免疫原
- Substance P and Full Lenght HRP
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亚型
- IgG1, IgG2a
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anti-Substance P antibody
适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Substance P (AA 58-68) antibody适用: 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Substance P antibody适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal SP-DE4-21 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 2 ml: IHC: 1:100 - 1:200. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized tissue culture supernatant
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Substance P
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背景
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Substance P, tachykinin, neuropeptide, neurokinin, SP, anti-tachykinin, anti-neuropeptide, anti-neurokinin, anti-SP
This antibody is a bispecific monoclonal antibody capable of simultaneously recognize the horseradish peroxidase (HRP) and Substance P. Bi-specific monoclonal antibodies have been successfully used in one step immunocytochemistry and immuno-dot assays. These results demonstrated that bispecific antibodies can be applied in quick and simple staining procedures and provide intense immunostaining. The P4C1 antibody has been successfully used in immunoassays and immunohistochemistry. Light and electron micrographs of rat tissue developed using this antibody clearly demonstrate substance P immunoreactive sites with very minimal non-specific background binding.
抗原
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