Recombinant PNKP 抗体
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北京 101111
Quick Overview for Recombinant PNKP 抗体 (ABIN7882001)
抗原
See all PNKP 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PNKP Antibody / Polynucleotide kinase 3 phosphatase
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human PNK / PNKP was used as the immunogen for the PNKP antibody.
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亚型
- IgG
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anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 60-320) antibody
PNKP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) antibodyPNKP 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) antibodyPNKP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB965 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB805 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-243) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 359-408) antibodyPNKP 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人, 小鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PNKP antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the PNKP antibody at -20oC.
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- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
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别名
- PNKP
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背景
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PNKP antibody detects polynucleotide kinase 3'-phosphatase, a DNA repair enzyme with dual kinase and phosphatase activities. PNKP processes DNA ends by phosphorylating 5' termini and dephosphorylating 3' termini, preparing them for ligation during single-strand and double-strand break repair. This enzyme functions in multiple repair pathways, including base excision repair and non-homologous end joining, ensuring genomic stability after DNA damage.
Research using PNKP antibody has revealed the protein's significance in neurodegenerative diseases and cancer. Mutations in PNKP cause neurological disorders such as microcephaly with seizures and developmental delay (MCSZ), where defective DNA repair leads to progressive neuronal loss. In oncology, PNKP is upregulated in certain tumors, where it contributes to resistance against radiation and chemotherapy by repairing therapy-induced DNA damage. Targeting PNKP is therefore a promising therapeutic strategy to sensitize tumors to genotoxic treatments.
PNKP also plays a role in maintaining mitochondrial DNA integrity, protecting cells from oxidative stress. Its activity is essential for neuronal survival and for preventing age-related genomic instability. Understanding PNKP regulation provides insight into how cells balance repair efficiency with the risk of mutagenesis.
Antibodies against PNKP are validated for use in western blot, immunofluorescence, immunoprecipitation, and immunohistochemistry. These tools allow researchers to track DNA repair activity, analyze protein localization in nuclei and mitochondria, and explore mechanisms of therapy resistance. Clone-based antibodies ensure reproducible results across studies.
NSJ Bioreagents supplies this PNKP antibody to support research in DNA repair, neurobiology, and cancer therapeutics. -
UniProt
- Q96T60
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途径
- DNA Damage Repair, Nucleotide Phosphorylation
抗原
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