Nucleolin 抗体
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北京 101111
Quick Overview for Nucleolin 抗体 (ABIN7881849)
抗原
See all Nucleolin (NCL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Nucleolin Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the anti-Nucleolin antibody.
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亚型
- IgG1, kappa
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyCarrier-free NCL 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 425-455) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) (AA 428-455) antibodyNCL 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17464 unconjugated
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应用备注
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Optimal dilution of the anti-Nucleolin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
- Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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