Nuclear Antigen 抗体
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Quick Overview for Nuclear Antigen 抗体 (ABIN7881829)
抗原
See all Nuclear Antigen (IPO-38) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- IPO38 Antibody (Proliferation Marker)
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纯化方法
- PEG precipitation
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免疫原
- Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO38 antibody.
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亚型
- IgM, kappa
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anti-Nuclear Antigen (IPO-38) antibody
IPO-38 适用: 人, 小鼠, 大鼠 FACS, IF, IHC 宿主: 小鼠 Monoclonal SPM260 unconjugated
anti-Nuclear Antigen (IPO-38) antibodyIPO-38 适用: 人, 小鼠, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal IPO-38 unconjugated
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应用备注
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Optimal dilution of the IPO38 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the IPO38 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Nuclear Antigen (IPO-38)
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别名
- IPO38
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背景
- Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.
抗原
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