Nuclear Antigen 抗体
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Quick Overview for Nuclear Antigen 抗体 (ABIN3025764)
抗原
See all Nuclear Antigen (IPO-38) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- IPO-38 Antibody (Proliferation Marker) (azide and preservative free)
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纯化方法
- PEG precipitation
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免疫原
- Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.
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亚型
- IgM, kappa
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anti-Nuclear Antigen (IPO-38) antibody
IPO-38 适用: 人, 小鼠, 大鼠 IHC, FACS, IF 宿主: 小鼠 Monoclonal SPM260 unconjugated
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应用备注
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Optimal dilution of the IPO-38 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the IPO-38 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Nuclear Antigen (IPO-38)
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别名
- IPO-38
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背景
- Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.
抗原
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