Nkx2-2 抗体
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北京 101111
Quick Overview for Nkx2-2 抗体 (ABIN7881814)
抗原
See all Nkx2-2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NKX2.2 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human recombinant protein was used as the immunogen for this NKX2.2 antibody.
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亚型
- IgG2b, kappa
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应用备注
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The optimal dilution of the NKX2.2 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))
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别名
- NKX2.2
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背景
- Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.
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UniProt
- O95096
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途径
- Dopaminergic Neurogenesis
抗原
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