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Laminin gamma 1 抗体

The 大鼠 单克隆 anti-Laminin gamma 1 antibody (Clone SPM193) (ABIN7881461) specifically detects Laminin gamma 1 in IHC (p). The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN7881461
发货至: 中国
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Quick Overview for Laminin gamma 1 抗体 (ABIN7881461)

抗原

See all Laminin gamma 1 (LAMC1) 抗体
Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

适用

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人, 小鼠

宿主

  • 68
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大鼠

克隆类型

  • 44
  • 39
单克隆

标记

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This Laminin gamma 1 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM193
  • 原理

    Laminin gamma 1 Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.

    亚型

    IgG2a, kappa
  • 应用备注

    Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Laminin gamma 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

    别名

    Laminin gamma 1

    背景

    Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.

    UniProt

    P11047
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