Recombinant Histone H1 抗体
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北京 101111
Quick Overview for Recombinant Histone H1 抗体 (ABIN7881065)
抗原
See all Histone H1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Histone H1 Antibody (azide and preservative free)
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纯化方法
- Protein A/G affinity
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免疫原
- Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone H1 antibody.
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亚型
- IgG2b, kappa
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应用备注
- Optimal dilution of the Histone H1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Aliquot the Histone H1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- Histone H1
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背景
- Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
抗原
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