Recombinant Histone H1 抗体
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北京 101111
Quick Overview for Recombinant Histone H1 抗体 (ABIN7881062)
抗原
See all Histone H1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Histone H1 Antibody
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纯化方法
- Protein A affinity chromatography
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免疫原
- Nuclei of human leukemia biopsy cells were used as the immunogen for this recombinant Histone H1 antibody.
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亚型
- IgG
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal 1415-1 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 (AA 1-105) antibody适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 WB, IF, IHC, FACS 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 WB, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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应用备注
- Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Histone H1
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背景
- Please note that this antibody is a recombinant rabbit version of original anti-histone H1 antibody (clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, this recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
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UniProt
- P07305
抗原
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