Recombinant ATG10 抗体
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北京 101111
Quick Overview for Recombinant ATG10 抗体 (ABIN7880028)
抗原
See all ATG10 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- ATG10 Antibody / Autophagy-related protein 10
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Apg10 (Atg10) was used as the immunogen for the ATG10 antibody.
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亚型
- IgG
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anti-Autophagy Related 10 (ATG10) (AA 1-125) antibody
ATG10 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 8A9B3 unconjugated
anti-Autophagy Related 10 (ATG10) (AA 1-220) antibodyATG10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 10 (ATG10) (AA 15-45), (N-Term) antibodyATG10 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB7550 unconjugated
anti-Autophagy Related 10 (ATG10) (AA 1-50) antibodyATG10 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 10 (ATG10) (AA 1-220) antibodyATG10 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 10 (ATG10) antibodyATG10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Autophagy Related 10 (ATG10) antibodyATG10 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4860 unconjugated Recombinant Antibody
anti-Autophagy Related 10 (ATG10) antibodyATG10 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 10 (ATG10) (C-Term) antibodyATG10 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 10 (ATG10) (C-Term) antibodyATG10 适用: 人, 犬, 兔, 马, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the ATG10 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the ATG10 antibody at -20oC.
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- ATG10 (Autophagy Related 10 (ATG10))
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别名
- ATG10
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背景
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ATG10 antibody detects Autophagy-related protein 10, an essential E2-like enzyme that catalyzes conjugation of ATG12 to ATG5, a key step in autophagosome formation. ATG10 plays a central role in the autophagy machinery by facilitating complex assembly required for membrane elongation and vesicle maturation. The ATG10 antibody is widely used in cell biology and metabolism research to study autophagy regulation, stress adaptation, and cellular degradation pathways.
ATG10 is encoded by the ATG10 gene located on human chromosome 5q14.1. The protein is approximately 220 amino acids long and resides in the cytoplasm, where it transiently associates with autophagic membranes. Functionally, ATG10 acts as an E2-like enzyme, transferring the ATG12 conjugate to ATG5 under the direction of E1-like enzyme ATG7. This conjugation system forms the ATG12-ATG5-ATG16L1 complex that defines the site of autophagosome formation.
The ATG10 antibody detects a 25 kilodalton band by western blot and shows diffuse cytoplasmic staining under confocal microscopy. ATG10 expression increases under nutrient deprivation and oxidative stress, linking metabolic signals to autophagy induction. Inhibition or depletion of ATG10 results in defective autophagosome biogenesis, accumulation of damaged organelles, and increased susceptibility to stress-induced apoptosis.
ATG10 also participates in noncanonical autophagy and immune signaling by modulating antigen presentation and cytokine secretion. Variations in ATG10 have been linked to cancers, inflammatory disorders, and aging-related diseases. Its regulation is tightly controlled through transcriptional activation by FOXO and ATF4, and by post-translational modifications that modulate enzyme activity.
Because of its indispensable role in the autophagy conjugation system, ATG10 provides a molecular link between stress sensing and degradative homeostasis. NSJ Bioreagents provides a validated ATG10 antibody optimized for its applications, supporting research into cellular degradation, metabolism, and survival mechanisms. -
UniProt
- Q9H0Y0
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途径
- ER-Nucleus Signaling, Autophagy
抗原
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