Recombinant HMGCL 抗体
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Quick Overview for Recombinant HMGCL 抗体 (ABIN7879850)
抗原
See all HMGCL 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HMGCL Antibody / Hydroxymethylglutaryl CoA lyase
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human HMGCL was used as the immunogen for the HMGCL antibody.
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亚型
- IgG
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anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (full length) antibody
HMGCL 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 1-325) antibodyHMGCL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F4-D1 unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) antibodyHMGCL 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 1-325) antibodyHMGCL 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 28-325) antibodyHMGCL 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 1-325) antibodyHMGCL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) antibodyHMGCL 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 81-130) antibodyHMGCL 适用: 人, 小鼠, 大鼠, 犬, 马, 豚鼠, Bat, Drosophila melanogaster, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 251-300) antibodyHMGCL 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (C-Term) antibodyHMGCL 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 斑马鱼, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the HMGCL antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the HMGCL antibody at -20oC.
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- HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))
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别名
- HMGCL
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背景
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HMGCL antibody detects Hydroxymethylglutaryl CoA lyase, encoded by the HMGCL gene. Hydroxymethylglutaryl CoA lyase is a mitochondrial enzyme involved in ketogenesis and leucine catabolism. HMGCL antibody provides researchers with a critical reagent to study energy metabolism, amino acid degradation, and inherited metabolic disorders.
Hydroxymethylglutaryl CoA lyase catalyzes the cleavage of HMG CoA into acetyl CoA and acetoacetate, a key step in ketone body production. Research using HMGCL antibody has shown that this enzyme provides alternative energy substrates during fasting, exercise, and carbohydrate restriction. By producing ketone bodies, Hydroxymethylglutaryl CoA lyase ensures energy supply for the brain, heart, and skeletal muscle when glucose availability is limited.
Studies with HMGCL antibody have demonstrated that the enzyme is also essential for leucine degradation. Defects in HMGCL impair leucine catabolism and ketone body production, resulting in HMG CoA lyase deficiency, a rare autosomal recessive metabolic disorder. Patients present with recurrent metabolic crises, hypoglycemia, metabolic acidosis, and lethargy. These findings underscore the importance of Hydroxymethylglutaryl CoA lyase in both amino acid metabolism and energy balance.
In addition to inherited disease, HMGCL function is relevant in cancer and diabetes. Research using HMGCL antibody has shown that alterations in ketone body metabolism influence tumor growth and metabolic adaptation. Some cancers reprogram ketone metabolism to support proliferation, while others are inhibited by high ketone levels. In diabetes, dysregulation of ketogenesis contributes to ketoacidosis, making HMGCL a critical enzyme for clinical monitoring and research.
Hydroxymethylglutaryl CoA lyase is localized to the mitochondrial matrix. Studies with HMGCL antibody have confirmed mitochondrial enrichment, with expression highest in liver and kidney, the major ketogenic organs. Tissue distribution reflects its role in systemic energy metabolism and adaptation to nutrient availability.
HMGCL antibody is widely applied in western blotting, immunohistochemistry, and enzymatic activity studies. Western blotting quantifies protein expression, immunohistochemistry identifies tissue distribution, and activity assays measure enzymatic function. These applications make HMGCL antibody indispensable for metabolic biology research.
By providing validated HMGCL antibody reagents, NSJ Bioreagents supports studies into ketogenesis, amino acid metabolism, and disease. Detection of Hydroxymethylglutaryl CoA lyase provides insights into how cells adapt to energy stress and how metabolic defects lead to disease. -
UniProt
- P35914
抗原
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