Recombinant EIF2AK2 抗体 (pThr451)
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Quick Overview for Recombinant EIF2AK2 抗体 (pThr451) (ABIN7879796)
抗原
See all EIF2AK2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr451
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原理
- Phospho-PKR (Thr451) Antibody / Protein kinase R / EIF2AK2
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-Phospho-PKR (T451) was used as the immunogen for the Phospho-PKR (Thr451) antibody.
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibodyEIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 329-551) antibodyEIF2AK2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6H3A10 unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 224-502) antibodyEIF2AK2 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) antibodyKD Validated EIF2AK2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB7125 unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibodyEIF2AK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibodyKD Validated EIF2AK2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1710 unconjugated Recombinant Antibody
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 519-550) antibodyEIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 11-42) antibodyEIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr446) antibodyEIF2AK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-PKR (Thr451) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-PKR (Thr451) antibody at -20oC.
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- EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
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别名
- PKR
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背景
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Phospho-PKR (Thr451) antibody detects protein kinase R phosphorylated at threonine 451. PKR, encoded by the EIF2AK2 gene, is a serine threonine kinase activated by double stranded RNA during viral infection. Upon activation, PKR undergoes autophosphorylation at Thr451 and other residues, leading to phosphorylation of eukaryotic initiation factor 2 alpha (eIF2alpha). This modification suppresses translation, limiting viral replication and regulating cellular stress responses.
Phospho-PKR (Thr451) antibody is widely applied in virology, immunology, and stress signaling research. By detecting PKR activation, researchers can evaluate how cells respond to viral infection and other stressors such as interferon signaling, oxidative stress, and endoplasmic reticulum stress. Phosphorylation at Thr451 provides a reliable marker of PKR kinase activity and initiation of antiviral defense pathways.
Western blot assays reveal phosphorylated PKR bands distinct from unphosphorylated protein. Immunohistochemistry highlights PKR activation in infected or stressed tissues, while immunofluorescence demonstrates subcellular localization of phospho PKR in cytoplasmic foci and stress granules. These methods provide detailed insight into antiviral signaling and translational control.
PKR phosphorylation at Thr451 integrates innate immunity and apoptosis. Persistent activation induces cell death, contributing to tissue pathology during infection and chronic disease. Dysregulation of PKR activity has been implicated in cancer, metabolic disorders, and neurodegenerative disease. By applying Phospho-PKR (Thr451) antibody, scientists can investigate the balance between protective antiviral signaling and detrimental chronic activation.
PKR also modulates inflammatory pathways including NF-kB activation and cytokine production. Through its ability to couple stress responses to immune activation, PKR influences outcomes in infection, autoimmunity, and tumor immunity. The phospho-specific antibody therefore provides a versatile tool for studying immune regulation and therapeutic targeting.
Phospho-PKR (Thr451) antibody from NSJ Bioreagents offers dependable specificity for monitoring EIF2AK2 activation, supporting high-quality research in virology, oncology, and immunology. -
UniProt
- P19525
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途径
- DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C
抗原
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