Recombinant CAMK2A 抗体 (pThr286)
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Quick Overview for Recombinant CAMK2A 抗体 (pThr286) (ABIN7879791)
抗原
See all CAMK2A 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr286
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原理
- Phospho-CaMKII alpha (T286) Antibody / CAMK2A
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human Phospho-CaMKII alpha (T286) was used as the immunogen for the Phospho-CaMKII alpha (T286) antibody. Due to the conserved nature of the epitope, the antibody will likely also detect Phospho-CaMKII beta (T287).
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亚型
- IgG
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anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibody
CAMK2A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C4 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibodyCAMK2A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyMicroarray verified CAMK2A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB443776 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 446-478), (C-Term) antibodyCAMK2A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1258 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibodyCAMK2A 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr306) antibodyCAMK2A 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-CaMKII alpha (T286) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-CaMKII alpha (T286) antibody at -20oC.
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- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
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别名
- CaMKII alpha
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背景
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Phospho-CaMKII alpha (T286) antibody detects calcium/calmodulin-dependent protein kinase II alpha when phosphorylated at threonine 286. The protein is encoded by the CAMK2A gene and is commonly referred to as CaM kinase II alpha, CaMK-II subunit alpha, and calcium/calmodulin-dependent protein kinase type II alpha chain. CaMKII alpha is a serine/threonine kinase expressed abundantly in the brain, where it regulates synaptic plasticity, learning, and memory. Autophosphorylation at threonine 286 converts the kinase into a calcium/calmodulin-independent active form, a hallmark event in long-term potentiation and neuronal signaling.
Phospho-CaMKII alpha (T286) antibody is widely applied in neuroscience, synaptic biology, and signaling research. Detecting phosphorylation at this site provides a direct measure of CaMKII activation, linking cellular activity to memory formation. In neurons, Thr286 phosphorylation prolongs kinase activity beyond transient calcium spikes, enabling sustained synaptic strengthening. By targeting this phosphorylation event, researchers can explore how activity-dependent signaling translates into lasting changes in connectivity.
Applications for Phospho-CaMKII alpha (T286) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated CaMKII alpha isoforms in brain lysates, immunohistochemistry maps activation in hippocampus and cortex, and immunofluorescence reveals localization at dendritic spines. These methods provide detailed views of synaptic signaling in vitro and in vivo.
Phosphorylation at Thr286 has been extensively studied in relation to long-term potentiation, a cellular mechanism of memory storage. Abnormal regulation of CaMKII phosphorylation contributes to neurological disorders, including Alzheimer disease, schizophrenia, and epilepsy. By applying Phospho-CaMKII alpha (T286) antibody, scientists can investigate disease-related disruptions in synaptic plasticity.
Beyond neuroscience, CaMKII alpha also participates in cardiac signaling and metabolism. Phosphorylation at Thr286 has been detected in cardiac stress responses and metabolic regulation. The antibody therefore supports research into CaMKII biology across systems. NSJ Bioreagents offers Phospho-CaMKII alpha (T286) antibody with validated specificity, ensuring reliable results in neuronal and disease studies. -
UniProt
- Q9UQM7
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途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
抗原
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