Recombinant ULK1 抗体 (pSer556)
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北京 101111
Quick Overview for Recombinant ULK1 抗体 (pSer556) (ABIN7879758)
抗原
See all ULK1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer556
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原理
- Phospho-ULK1 (Ser556) Antibody / Unc-51 like autophagy activating kinase 1
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human Phospho-ULK1 (S556) was used as the immunogen for the Phospho-ULK1 (Ser556) antibody.
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亚型
- IgG
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anti-Unc-51 Like Kinase 1 (ULK1) (AA 951-1050) antibody
ULK1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 602-715) antibodyULK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) antibodyULK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (pSer556) antibodyULK1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (Atto 390)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 390
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (Atto 488)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 488
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (HRP)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (FITC)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (Atto 594)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 594
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (Biotin)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
- Optimal dilution of the Phospho-ULK1 (Ser556) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-ULK1 (Ser556) antibody at -20oC.
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- ULK1 (Unc-51 Like Kinase 1 (ULK1))
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别名
- ULK1
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背景
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Phospho-ULK1 (Ser556) antibody detects Unc-51 like autophagy activating kinase 1 phosphorylated at serine 556, encoded by the ULK1 gene. ULK1 is a serine/threonine kinase that initiates autophagy in response to nutrient status and energy stress. Phosphorylation at Ser556 is an important regulatory event that integrates upstream signaling pathways to control autophagosome formation. Phospho-ULK1 (Ser556) antibody provides researchers with a highly specific reagent to study the regulation of autophagy and its role in physiology and disease.
ULK1 functions as the mammalian homolog of yeast Atg1, acting at the top of the autophagy hierarchy. Research using Phospho-ULK1 (Ser556) antibody has demonstrated that phosphorylation at serine 556 by AMP activated protein kinase promotes activation of ULK1 under conditions of energy depletion. This modification stimulates autophagy initiation by enabling the ULK1 complex to recruit downstream autophagy machinery. By contrast, mTORC1 signaling suppresses ULK1 through phosphorylation at other sites, highlighting the dynamic control of ULK1 activity by nutrient and energy signals.
Phospho-ULK1 (Ser556) antibody has been used to show that phosphorylation at this site acts as a molecular switch for autophagy induction. In starvation or low energy states, phosphorylation at Ser556 ensures cells adapt by recycling cytoplasmic contents through lysosomal degradation. This process provides metabolic substrates for survival and maintains homeostasis. Inhibiting phosphorylation at Ser556 impairs autophagy, underscoring its essential role in this pathway.
Dysregulation of ULK1 phosphorylation has been linked to human disease. Studies with Phospho-ULK1 (Ser556) antibody have revealed that impaired autophagy contributes to neurodegenerative diseases, metabolic disorders, and cancer. In cancer, altered ULK1 phosphorylation influences tumor cell survival under stress conditions, promoting growth in nutrient-poor environments. In neurodegeneration, reduced ULK1 activation leads to accumulation of toxic proteins. These findings emphasize the clinical importance of monitoring ULK1 phosphorylation states.
Phospho-ULK1 (Ser556) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals phosphorylation-dependent shifts in ULK1 activity, immunohistochemistry highlights tissue-specific patterns of autophagy activation, and immunofluorescence demonstrates dynamic localization of phosphorylated ULK1 at autophagosome initiation sites. These applications make Phospho-ULK1 (Ser556) antibody an indispensable reagent in autophagy research.
By supplying validated Phospho-ULK1 (Ser556) antibody reagents, NSJ Bioreagents supports research into energy sensing, autophagy regulation, and disease mechanisms. Detection of phosphorylation at Ser556 provides a precise marker of ULK1 activation and autophagy initiation. -
UniProt
- O75385
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途径
- Regulation of Cell Size, Autophagy
抗原
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