Recombinant NeuN 抗体 (full length)
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北京 101111
Quick Overview for Recombinant NeuN 抗体 (full length) (ABIN7878933)
抗原
See all NeuN (RBFOX3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- Recombinant NeuN Antibody / Fox3
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纯化方法
- Protein A/G affinity
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免疫原
- Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody.
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亚型
- IgG, kappa
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应用备注
- Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the recombinant NeuN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
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别名
- NeuN
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背景
- RNA binding protein fox-1 homolog 3, also called RBFOX3, Neuronal Nuclei Antigen and NeuN, is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
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UniProt
- A6NFN3
抗原
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