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Recombinant NeuN 抗体 (full length)

The 兔 单克隆 anti-NeuN antibody is suitable to detect NeuN in samples from 人. It has been validated for WB 和 IHC (p).
产品编号 ABIN7878933
发货至: 中国
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Quick Overview for Recombinant NeuN 抗体 (full length) (ABIN7878933)

抗原

See all NeuN (RBFOX3) 抗体
NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This NeuN antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

NeuN-288R
  • 抗原表位

    • 20
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    full length

    原理

    Recombinant NeuN Antibody / Fox3

    纯化方法

    Protein A/G affinity

    免疫原

    Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody.

    亚型

    IgG, kappa
  • 应用备注

    Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot the recombinant NeuN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
  • 抗原

    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

    别名

    NeuN

    背景

    RNA binding protein fox-1 homolog 3, also called RBFOX3, Neuronal Nuclei Antigen and NeuN, is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

    UniProt

    A6NFN3
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