PLAP 抗体 (full length)
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北京 101111
Quick Overview for PLAP 抗体 (full length) (ABIN7878815)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- PLAP Antibody / Placental Alkaline Phosphatase
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human ALPP/placental alkaline phosphatase protein was used as the immunogen for the PLAP antibody.
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亚型
- IgG2b, kappa
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应用备注
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Optimal dilution of the PLAP antibody should be determined by the researcher.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PLAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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别名
- PLAP
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背景
- Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
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UniProt
- P05187
抗原
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