Recombinant Nucleolin 抗体 (full length)
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Quick Overview for Recombinant Nucleolin 抗体 (full length) (ABIN7878738)
抗原
See all Nucleolin (NCL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- full length
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原理
- Nucleolin Antibody / NCL (azide and preservative free)
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纯化方法
- Protein A/G affinity
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免疫原
- Recombinant full-length human NCL protein was used as the immunogen for the Nucleolin antibody.
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亚型
- IgG, kappa
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应用备注
- Optimal dilution of the Nucleolin antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
- Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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