PIK3C3 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for PIK3C3 抗体 (C-Term) (ABIN7877455)
抗原
See all PIK3C3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
原理
- PIK3C3 Antibody / VPS34 / Phosphatidylinositol 3-kinase catalytic subunit type 3
-
纯化方法
- Immunogen affinity purified
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human VPS34/PIK3C3 was used as the immunogen for the PIK3C3 antibody.
-
亚型
- IgG
-
-
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 631-885) antibody
PIK3C3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 14-39) antibodyPIK3C3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11895 unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (pSer164) antibodyPIK3C3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (pSer282) antibodyPIK3C3 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) antibodyKD Validated PIK3C3 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 24GB1150 unconjugated Recombinant Antibody
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 24-53), (N-Term) antibodyPIK3C3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB1695 unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 301-400) antibodyPIK3C3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 700-850), (Catalytic Subunit) antibodyPIK3C3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 150-250) antibodyPIK3C3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 770-801), (C-Term) antibodyPIK3C3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB10857 unconjugated
-
-
应用备注
- Optimal dilution of the PIK3C3 antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- After reconstitution, the PIK3C3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
-
-
- PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))
-
别名
- PIK3C3
-
背景
-
PIK3C3 antibody detects Phosphatidylinositol 3-kinase catalytic subunit type 3, a lipid kinase that generates phosphatidylinositol 3-phosphate (PI3P) to control autophagy and endosomal trafficking. The UniProt recommended name is Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), also known as Vacuolar protein sorting-associated protein 34 (VPS34). This enzyme is the only class III PI3K in mammalian cells and plays a pivotal role in autophagosome formation and lysosomal function.
Functionally, PIK3C3 antibody recognizes a 887-amino-acid cytoplasmic protein that catalyzes the phosphorylation of phosphatidylinositol to generate PI3P, recruiting downstream effectors such as WIPI and DFCP1. PIK3C3 forms multiprotein complexes with Beclin 1, UVRAG, ATG14, and VPS15 to regulate membrane nucleation during autophagy and endosome maturation. Its lipid kinase activity is crucial for endocytic trafficking, nutrient sensing, and organelle biogenesis.
The PIK3C3 gene, located on chromosome 18q12.3, is ubiquitously expressed and evolutionarily conserved from yeast to humans. It governs autophagic flux by mediating vesicle formation and fusion with lysosomes. Through its interaction with Beclin 1, PIK3C3 serves as a core regulator of cellular homeostasis under nutrient stress and is involved in tumor suppression and neuroprotection.
Pathologically, dysregulation of PIK3C3/VPS34 contributes to cancer, neurodegeneration, and infectious disease. Inhibition of VPS34 impairs autophagy, leading to protein aggregation and metabolic imbalance. Conversely, enhanced PIK3C3 activity supports tumor cell survival under nutrient deprivation. The enzyme is also hijacked by viruses to generate replication compartments. Research using PIK3C3 antibody advances understanding of autophagic control, endosomal transport, and signaling adaptation to stress.
PIK3C3 antibody is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect VPS34 and its complexes. It supports studies in autophagy, intracellular trafficking, and lipid signaling. NSJ Bioreagents provides validated PIK3C3 antibody reagents optimized for autophagy and vesicle biogenesis research.
Structurally, PIK3C3 consists of a helical domain, a C2 domain for membrane association, and a lipid kinase catalytic domain. Its activity depends on interactions with VPS15 and Beclin 1 for stabilization and subcellular localization. This antibody facilitates analysis of PIK3C3/VPS34's central role in autophagy initiation and endolysosomal trafficking. -
UniProt
- Q8NEB9
-
途径
- AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy
抗原
-
