PAM 抗体 (AA 69-914)
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北京 101111
Quick Overview for PAM 抗体 (AA 69-914) (ABIN7876451)
抗原
See all PAM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 69-914
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原理
- PAM Antibody / Peptidyl-glycine alpha-amidating monooxygenase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PAM recombinant protein (Position: K69-R914) was used as the immunogen for the PAM antibody.
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亚型
- IgG
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anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (AA 338-497) antibody
PAM 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (AA 21-288) antibodyPAM 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) antibodyPAM 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB15840 unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) antibodyPAM 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (C-Term) antibodyVerified PAM 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (Internal Region) antibodyPAM 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (AA 146-195) antibodyPAM 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Peptidylglycine alpha-Amidating Monooxygenase (PAM) (N-Term) antibodyPAM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PAM antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
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别名
- PAM
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背景
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PAM antibody detects Peptidyl-glycine alpha-amidating monooxygenase, encoded by the PAM gene on chromosome 5q23.2. PAM antibody is used to study this bifunctional enzyme that catalyzes peptide amidation, a modification essential for the activity of many neuropeptides and hormones. Amidation increases peptide stability, receptor affinity, and biological potency. PAM contains two catalytic domains: peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). PHM hydroxylates the terminal glycine of a peptide substrate, and PAL then cleaves the hydroxylated intermediate to generate the amidated, active peptide.
PAM expression is highest in neuroendocrine tissues including brain, pituitary, adrenal glands, and pancreas, where it activates signaling molecules like vasopressin, oxytocin, gastrin, and calcitonin. By processing these hormones and neuropeptides, PAM regulates stress response, water homeostasis, appetite, metabolism, and cardiovascular activity. Because the enzyme requires copper, ascorbate, and molecular oxygen, its activity is tied to nutritional status and redox balance. PAM localization to secretory granules ensures amidation occurs during peptide packaging and secretion.
Disruption of PAM activity impairs peptide amidation and alters neuroendocrine signaling. Knockout mice exhibit growth retardation, defective stress hormone responses, and metabolic dysregulation. In humans, variants in PAM have been associated with endocrine disorders, altered glucose regulation, and susceptibility to neuroendocrine tumors. Overexpression of PAM in tumors may enhance production of amidated peptides that drive proliferation. PAM antibody is essential for studying how peptide hormones are matured and how dysregulated amidation contributes to disease.
Experimentally, PAM antibody is used in western blotting to detect isoforms, in immunohistochemistry to visualize tissue distribution, and in immunofluorescence to highlight localization in secretory granules. Co-immunoprecipitation with PAM antibody identifies interactions with granin proteins and vesicle transport machinery. Functional assays pair PAM antibody with peptide amidation measurements to connect enzyme expression with biological activity. NSJ Bioreagents offers PAM antibody to support research in neuroendocrinology, peptide biology, and tumor biology. -
UniProt
- P19021
抗原
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