PTCD3 抗体 (AA 66-648)
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Quick Overview for PTCD3 抗体 (AA 66-648) (ABIN7876349)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 66-648
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原理
- PTCD3 Antibody / Pentatricopeptide repeat domain-containing protein 3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PTCD3 recombinant protein (Position: D66-R648) was used as the immunogen for the PTCD3 antibody.
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亚型
- IgG
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anti-Pentatricopeptide Repeat Domain 3 (PTCD3) (AA 450-689) antibody
PTCD3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pentatricopeptide Repeat Domain 3 (PTCD3) antibodyPTCD3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Pentatricopeptide Repeat Domain 3 (PTCD3) (AA 1-689) antibodyPTCD3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pentatricopeptide Repeat Domain 3 (PTCD3) (C-Term) antibodyPTCD3 适用: 人, 小鼠, 大鼠, 马, 兔, Cow, 犬, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PTCD3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PTCD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PTCD3 (Pentatricopeptide Repeat Domain 3 (PTCD3))
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别名
- PTCD3
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背景
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PTCD3 antibody detects Pentatricopeptide repeat domain-containing protein 3, encoded by the PTCD3 gene on chromosome 2p11.2. PTCD3 antibody is used to study this mitochondrial RNA-binding protein required for mitochondrial translation and respiratory function. PTCD3 belongs to the large pentatricopeptide repeat (PPR) protein family, which regulates RNA metabolism in organelles. It is localized to the mitochondrial matrix, where it participates in ribosome assembly and translation initiation for mitochondrial-encoded genes. Expression is ubiquitous but highest in tissues with high metabolic demand such as heart, brain, and muscle.
Structurally, PTCD3 contains multiple PPR motifs that form helix-turn-helix repeats mediating RNA binding. These motifs allow PTCD3 to recognize specific RNA sequences, stabilizing ribosome-mRNA complexes and supporting efficient translation. PTCD3 associates with the small subunit of the mitochondrial ribosome (28S subunit), acting as a structural and functional component of the translation machinery.
Functionally, PTCD3 ensures proper mitochondrial protein synthesis by stabilizing ribosomal complexes and supporting initiation of translation. Knockdown or mutation disrupts translation of mitochondrial mRNAs, leading to impaired oxidative phosphorylation complex assembly and respiratory chain dysfunction. PTCD3 is also thought to regulate specific RNA processing events, although its exact targets remain under investigation. Researchers use PTCD3 antibody to investigate mitochondrial translation, ribosome biology, and energy metabolism.
Clinically, mutations in PTCD3 have been associated with mitochondrial encephalopathy, a disorder characterized by neurological dysfunction, muscle weakness, and lactic acidosis. PTCD3 deficiency results in combined oxidative phosphorylation defects, underscoring its essential role in mitochondrial function. Beyond inherited disorders, PTCD3 expression changes have been reported in cancers, reflecting the importance of mitochondrial activity in tumor metabolism. NSJ Bioreagents offers PTCD3 antibody for studies in mitochondrial biology, disease pathogenesis, and metabolic regulation.
Experimentally, PTCD3 antibody is applied in western blotting to detect the ~79 kDa protein, in immunofluorescence microscopy to confirm mitochondrial localization, and in immunohistochemistry to study tissue-specific expression. Immunoprecipitation with PTCD3 antibody allows isolation of mitochondrial ribosome complexes for functional analysis. -
UniProt
- Q96EY7
抗原
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